Fig. 3From: Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coliComparison of the effects of OsCOMT and SaCOMT on melatonin production. Two E. coli strains (10 OD) were harvested after 16 h of induction at 16 °C, resuspended in 1 mL pH 7.0 Tris–HCl, and cultured with 1 mg/mL NAS and 2 mg/mL methionine at 30 °C. Data are the means ± standard deviations of triplicate experiments. The absence of error bars indicates that the error was smaller than the symbol sizeBack to article page