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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coli

Fig. 2

Prokaryotic P4Hs catalyzed the formation of 5-HTP in E. coli. a The biosynthetic pathway of 5-HTP constructed in E. coliΔtnaA. b Partial results of P4H alignment. The sequences and functional domains of P4Hs were highly conserved, and the arrows indicate the site requiring the mutation of tryptophan to phenylalanine. c Effect of P4H on 5-HTP production in recombinant Escherichia coli. 5-HTP levels were subjected to HPLC, and the data are the means ± standard deviations of triplicate experiments

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Microbial Cell Factories

ISSN: 1475-2859

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