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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Improvement of FK506 production via metabolic engineering-guided combinational strategies in Streptomyces tsukubaensis

Fig. 4

FK506 biosynthetic gene cluster and analysis of FK506 production and relative expression levels. a Proposed organization of the transcription units ( →) in FK506 biosynthetic gene cluster. b Effect on FK506 production by overexpressing tcsA/B/C/D (L19-3), fkbG/H/I/J/K (L19-4) or fkbO (L19-5) and in-situ substitution of the intergenic region between the gene fkbB and fkbO by constructed promoter cassettes (L19-6 and L19-7). c Relative expression levels of selected genes in S. tsukubaensis L19-6 and L19-7 compared with L19-2. Excluding genes located in the FK506 biosynthetic gene cluster, genes residing upstream and downstream regions of the FK506 biosynthetic gene cluster were also included. Each experiment was repeated for three times and error bars showed standard deviations. The asterisks indicated the statistic significant differences compared with L19-2, respectively. ****P < 0.0001; N, no significant difference

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