Table 1 Strains and plasmids used in this work
Name | Genetic characteristics | From |
|---|---|---|
Aspergillus niger CGMCC 10142 | CA producer, parental strain | The laboratory |
Aspergillus niger 20-15 | PglaA induced HGT1 overexpression strain, hygr | This work |
Aspergillus niger 21-8 | Paox1 induced HGT1 overexpression strain, hygr | This work |
E. coli DH5α | plasmid propagation | The laboratory |
A. tumefaciens AGL1 | plasmid transformation | The laboratory |
pGM-HGT1 | T-vector with A. niger HGT1 DNA, Ampr | This work |
p80-HSVtk | ku70 gene knockout plasmid, hygr Kanr | The laboratory |
p20 | HGT1 overexpression plasmid with promoter PglaA, hygr Kanr | This work |
p21 | HGT1 overexpression plasmid with promoter Paox1, hygr Kanr | This work |