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Table 2 Strains constructed in this study

From: Improved production of 2′-fucosyllactose in engineered Saccharomyces cerevisiae expressing a putative α-1, 2-fucosyltransferase from Bacillus cereus

Strain

Description

Reference or Source

FL01

W303-1a with pRS304-Pgal1-lac12-Tcyc1

This study

FL02

FL01 with pRS425-Pgal1-lac4-Tcyc1

This study

FL03

FL01 with pRS306-Pgal1-gmd-Tcyc1&Pgal10-wcaG-Tadh1

This study

FL04

FL03 with pRS305-Pgal1-futC (from H. pylori)- Tcyc1

This study

FL05

FL03 with pRS305-Pgal1-futAs (from Acetobacter sp.)- Tcyc1

This study

FL06

FL03 with pRS305-Pgal1-futBc (from B. cereus)- Tcyc1

This study

FL07

FL03 with pRS305-Pgal1-futBe (from B. eggerthii)- Tcyc1

This study

FL08

FL03 with pRS305-Pgal1-futBu (from B. unifomis)- Tcyc1

This study

FL09

FL03 with pRS305-Pgal1-futNc (from N. californiae)- Tcyc1

This study

FL16

FL06 with pRS303-Pgal1-sec53-Tcyc1&Pgal10-psa1-Tadh1

This study

FL17

FL16 with pUMRI-A-Pgal10-pmi40-Tadh1

This study

FL18

FL16 with pUMRI-A-Pgal1-spos5-Tcyc1&Pgal10-pmi40-Tadh1

This study

FL19

FL16 without Gal80

This study