Table 1 2′-FL production in different engineered organisms

E. coli

de novo and salvage pathway; FutC from H. pylori

20.28 ± 0.83 g/L

309.16 ±

12.61 mg/g CDW

[12]

E. coli

salvage pathway; FutC from H. pylori, FucI is deleted

23.1g/L

0.37 mole/mole lactose

[14]

E. coli

salvage pathway; WcfB from Bacteroides fragilis

15.4 g/L

0.858 g/g

lactose

[17]

E. coli

de novo pathway; FutC from H. pylori; deleting Ion and WcaJ; overexpressing Zwf and PntAB

9.12 g/L

Data not shown

[16]

E. coli

salvage pathway; FutC from H. pylori; deleting FucI, araA and rhaA

47.0 g/L

0.52mole 2-FL/mole fucose

[13]

E. coli

de novo pathway; α-1, 2-FT from Thermosynechococcus elongatus

0.49 g/L

Data not shown

[28]

B. subtilis

salvage pathway; FutC from H. pylori; enhancing GDP regeneration

5.01 g/L

0.85 mol/mol fucose

[18]

S. cerevisiae

salvage pathway; FutC from H. pylori

503 mg/L

0.44 mol/mol

from l-fucose, 0.25 mol/mol from lactose

[21]

S. cerevisiae

de novo pathway; FutC from H. pylori

0.51 g/L

0.229 mol/mol from lactose

[19]

S. cerevisiae

de novo pathway; FutC from H. pylori; SUMO-tagged-FutC; CDT2 transporter

15 g/L

~ 0.156 g/g CDW

[20]

S. cerevisiae

de novo pathway; WbgL from E. coli; utilizing xylose; Extra copies of 2′-FL synthetic genes

25.5 g/L

0.82 mol/mol from lactose

[23]

S. cerevisiae

de novo pathway; FutBc from B. cereus; overexpressing Sec53 and Psa1.

26.63 g/L

0.85 mol/mol from lactose

This study