Fig. 5

Deletion of Gal80 enabled 2′-FL production with sucrose as the carbon source. Gal80 was knockout in FL16, resulting strain FL19. The strains were cultured in YP medium containing glucose 2% (w/v) and 0.12 g/L adenine. Galactose 3% (w/v) or sucrose 3% (w/v) and lactose 0.4% (w/v) were added at 24 h and cultured for another 48 h. FL16 gal and FL19 gal indicate 2′-FL production from galactose while FL16 suc and FL19 suc indicate 2′-FL production from sucrose