From: Algal glycobiotechnology: omics approaches for strain improvement
Strain | Analytical method | Target product | Key findings | References |
---|---|---|---|---|
Chlorella vulgaris | MALDI-TOF MS, LC-ESI-MS | N-glycans | Analysis of N-glycosylation pathway in Chlorella vulgaris | [50] |
Chlamydomonas reinhardti | MALDI-TOF-MS | N-glycans | Analysis of N-glycosylation Pathway in Chlamydomonas reinhardtii | [51] |
Chlamydomonas reinhardtii | LC-ESI-MS | N-glycans | Reexamination of the N-glycosylation pathway in Chlamydomonas reinhardtii | [52] |
Chlamydomonas reinhardtii | LC-ESI-MS | N-glycans | An additional xylosyl transferase is connected with the xylosylation of protein N-linked glycans | [53] |
Phaeodactylum tricornutum | MALDI-TOF–MS | N-glycans, LLO | Study of lipid-linked oligosaccharides | [55] |
Porphyridium sp. | MALDI-TOF MS, MS/MS | N-glycans | The structural analysis of the N-glycans in the 66-kDa glycoprotein | [54] |
Phaeodactylum tricornutum | LC-ESI-MS | N-glycans | Turning out recombinant proteins using P. tricornutum with high mannose-type N-glycans condition | [56] |
Phaeodactylum tricornutum | MALDI-TOF/TOF | N-glycans | Over expression of the FuT54599 leads to an increase of the α (1,3)-fucosylation of the diatom endogenous glycoproteins | [57] |
Chlamydomonas reinhardtii | ESI-MS | O-glycans | Analysis of the O-glycosylation pathway in C. reinhardti | [58] |
Chlamydomonas reinhardtii | MALDI-TOF-MS. Ma | O-glycans | Genes encoding the serine O-α-galactosyltransferase were separated from Chlamydomonas reinhardtii | [59] |
Euglena gracilis | LC-ESI-MS | N-glycans, O-glycans | Euglena has a complex glycan surface | [63] |
Chlorella vulgaris | GCMS, LC-MS/MS and MN (Molecular networking) | Metabolite of C.vulgaris using metabolomics including glycolipid | Finding the presence of glycolipid via novel bioinformatics approaches such as the molecular networking | [60] |
Scytonema sp. UIC 10,036, and Nostoc sp. UIC 10,110 | LC-HRMS, XCMS Online | Primary metabolites (heterocyst glycolipids) | Lower nitrate levels inducing increased amounts of heterocyst glycolipids | [62] |
Chlorella UMACC050 | LC-MS analysis | Lipid (including glycolipid) | Lipid accumulation including glycolipid and enhancement of glycolytic activity induced by nitrogen depletion | [64] |