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Table 2 Metabolomics studies of microalgae: application of glycobiotechnology

From: Algal glycobiotechnology: omics approaches for strain improvement

Strain

Analytical method

Target product

Key findings

References

Chlorella vulgaris

MALDI-TOF MS, LC-ESI-MS

N-glycans

Analysis of N-glycosylation pathway in Chlorella vulgaris

[50]

Chlamydomonas reinhardti

MALDI-TOF-MS

N-glycans

Analysis of N-glycosylation Pathway in Chlamydomonas reinhardtii

[51]

Chlamydomonas reinhardtii

LC-ESI-MS

N-glycans

Reexamination of the N-glycosylation pathway in Chlamydomonas reinhardtii

[52]

Chlamydomonas reinhardtii

LC-ESI-MS

N-glycans

An additional xylosyl transferase is connected with the xylosylation of protein N-linked glycans

[53]

Phaeodactylum tricornutum

MALDI-TOF–MS

N-glycans, LLO

Study of lipid-linked oligosaccharides

[55]

Porphyridium sp.

MALDI-TOF MS, MS/MS

N-glycans

The structural analysis of the N-glycans in the 66-kDa glycoprotein

[54]

Phaeodactylum tricornutum

LC-ESI-MS

N-glycans

Turning out recombinant proteins using P. tricornutum with high mannose-type N-glycans condition

[56]

Phaeodactylum tricornutum

MALDI-TOF/TOF

N-glycans

Over expression of the FuT54599 leads to an increase of the α (1,3)-fucosylation of the diatom endogenous glycoproteins

[57]

Chlamydomonas reinhardtii

ESI-MS

O-glycans

Analysis of the O-glycosylation pathway in C. reinhardti

[58]

Chlamydomonas reinhardtii

MALDI-TOF-MS. Ma

O-glycans

Genes encoding the serine O-α-galactosyltransferase were separated from Chlamydomonas reinhardtii

[59]

Euglena gracilis

LC-ESI-MS

N-glycans, O-glycans

Euglena has a complex glycan surface

[63]

Chlorella vulgaris

GCMS, LC-MS/MS and MN (Molecular networking)

Metabolite of C.vulgaris using metabolomics including glycolipid

Finding the presence of glycolipid via novel bioinformatics approaches such as the molecular networking

[60]

Scytonema sp. UIC 10,036, and Nostoc sp. UIC 10,110

LC-HRMS, XCMS Online

Primary metabolites (heterocyst glycolipids)

Lower nitrate levels inducing increased amounts of heterocyst glycolipids

[62]

Chlorella UMACC050

LC-MS analysis

Lipid (including glycolipid)

Lipid accumulation including glycolipid and enhancement of glycolytic activity induced by nitrogen depletion

[64]