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Table 4 Experimental CCD design matrices of the variables with various C:N ratio and their response values of biomass yield, lipid yield, and lipid content of C. tropicalis ASY2 grown in ALB

From: Bioconversion of sago processing wastewater into biodiesel: Optimization of lipid production by an oleaginous yeast, Candida tropicalis ASY2 and its transesterification process using response surface methodology

Run

A:Starch, g L–1

B:Yeast extract, g L–1

C: Airflow rate,

L min–1

Biomass yield,

g L–1

Lipid yield, g L–1

Lipid content, %

1

20

0.5

3

3.56

1.66

46.63

2

15

0.75

5

5.56

2.42

43.53

3

20

0.5

7

6.89

2.71

39.33

4

15

0.75

5

5.52

2.23

40.4

5

15

0.75

5

5.78

2.32

40.14

6

15

0.75

5

5.46

2.25

41.21

7

6.59

0.75

5

2.21

0.35

15.84

8

10

1

3

3.04

0.79

25.99

9

15

0.75

5

4.92

2.19

44.51

10

10

1

7

6.21

1.31

21.1

11

10

0.5

3

2.94

0.83

28.23

12

20

1

3

4.18

1.56

37.32

13

15

1.17

5

6.98

2.15

30.8

14

15

0.75

5

5.34

2.42

45.32

15

20

1

7

8.25

1.65

20

16

15

0.75

8.36

8.05

1.62

20.12

17

15

0.75

1.64

2.05

0.61

29.76

18

23.41

0.75

5

5.13

2.11

41.13

19

10

0.5

7

5.12

1.45

28.32

20

15

0.33

5

5.45

2.84

52.11