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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Efficient expression of heterologous genes by the introduction of the endogenous glyceraldehyde-3-phosphate dehydrogenase gene intron 1 in Ganoderma lucidum

Fig. 2

Selection of phosphinothrin-resistant transformants on a selective CYM plate. A Transformants on a selective CYM plate. 1, 2, 3: the strain transformed with pJW-EXP-opbar-flag; 4, 5, 6: the strain transformed with pJW-EXP-in-opbar-flag. B Identificaton of G. lucidum transformants by PCR. Lane M, DNA marker DL5000; Lane P, pJW-EXP-in-opbar-flag as positive control; Lane N, negative control; Lane 1, 2, 3, the transformants pJW-EXP-opbar-flag; Lane 4, 5, 6, the transformant pJW-EXP-in-opbar-flag

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