Microbial Cell Factories

Table 1 Lists of homologous recombinant knockout and knockin vectors

From: Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria

Mutation	Vector	Targeted gene	Starting strain
KO	p2NIL-SacB-ΔKstD211	kstd211	WT
KO	p2NIL-Sacb-ΔKshA226	kshA226	HGMS2^Δkstd211
KO	p2NIL-SacB-ΔKshA395	kshA395	HGMS2^Δkstd211
KO	p2NIL-SacB-ΔKshB122	kshB122	HGMS2^Δkstd211
KI	p2NIL-SacB-KstD2	kstD211	HGMS2^Δkstd211 ^ΔkshB122
KI	p2NIL-SacB-KshA51	kshA226	HGMS2^Δkstd211

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ISSN: 1475-2859

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