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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria

Fig. 3

Knockout of the kstd and ksh genes from the HGMS2 strain. a A homologous recombination vector was constructed based on p2NIL plasmid. U: the upstream sequence; D: the downstream sequence. b PCR confirmation of putative kstd-knockout colonies, in comparison with that for the WT strain. M: DNA marker; WT: PCR products amplified from the HGMS2; ΔKstD211: two putative kstd-default mutants. c–e PCR confirmation of putative ksh-knockout colonies, in comparison with that for the WT strain. M: DNA marker; ΔKshA226: a putative HGMS2ΔkstD211 + ΔkshA226 mutant; KOA226-Pl: the plasmid p2NIL-Sac-ΔKshA226; WT: the HGMS2 strain; ΔKshA395: a putative HGMS2Δkstd211 + ΔkshA395 mutant; KOA395-Pl: the plasmid p2NIL-Sac-ΔKshA395; ΔKshB122a and ΔKshB122: two putative HGMS2Δkstd211 + ΔkshB122 mutants; KOB122-Pl: the plasmid p2NIL-Sac-ΔKshB122

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Microbial Cell Factories

ISSN: 1475-2859

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