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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria

Fig. 1

KstD and Ksh enzymes are responsible for 4-AD accumulation and its degradation during phytosterol catabolism in M. neoaurum HGMS2. The steroidal core structure contains four rings, namely, rings A, B, C, and D, labeled on phytosterol. Successive steps involve the formation of 9α-hydroxy-1,4-androstadiene-3,17-dione (9OH-ADD), which is a transient intermediate and simultaneously converts to 3-hydroxy-9,10-secoandrost-1,3,5(10)-triene-9,17-dione (HSA). 4-AD: 4-androstene-3,17-dione; ADD: 1,4-androstadiene-3,17-dione; 9OH-AD: 9α-hydroxy-4-androstene-3,17-dione and BA: 21-hydroxy-20-methylpregn-4-en-3-one

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Microbial Cell Factories

ISSN: 1475-2859

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