Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Combining functional metagenomics and glycoanalytics to identify enzymes that facilitate structural characterization of sulfated N-glycans

Fig. 5

F1-ORF13 sulfatase specificity. A Summary of the specificity of F1-ORF13 on monosaccharide substrates. Monosaccharides are represented using SNFG nomenclature [69]. A red crossed arrow indicates no activity of F1-ORF13 on these substrates. B, C Activity of in vitro expressed F1-ORF13 sulfatase on GlcNAc-6-SO4 (B) and GalNAc-6-SO4 (C). Analysis was performed by high-performance anion-exchange chromatography with pulsed amperometric detection. Reactions were performed in duplicate (F1-ORF13-A and -B – blue chromatograms). Control reactions consists of in vitro expression in the absence of F1-ORF13 (grey chromatogram). Sulfated and non-sulfated monosaccharides dissolved in water were run as retention time standards (black chromatograms)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us