Fig. 3

Function- and sequence-based analysis of fosmids F1-F12. A Rescreening analysis of fosmids F1-F12. Fosmids F1-F12 were tested using four distinct assays: using 4MU-GlcNAc-6-SO₄ with exogenous hexosaminidase (GlcNAc-SO₄ + Hex), 4MU-GlcNAc-6-SO₄ in the absence of exogenous enzyme (GlcNAc-SO₄), asulfated 4MU-GlcNAc (GlcNAc), and 4MU-SO₄ (SO₄). For each fosmid, shown is its activity in each assay, the predicted number of sulfatase and hexosaminidase genes found within its DNA sequence, the closest species of origin as determined by BLAST against the entire NCBI nucleotide collection, and the size of the cloned insert. B Sequence alignment of fosmids F1-F12. A Circos plot made with Circoletto software illustrates relatedness in the DNA sequences of the 12 clones. Ribbons are colored using BLAST bitscores within Circoletto