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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona

Fig. 3

The differences in growth and sporulation between the wild-type and mutants on CSM medium. a The difference in growth of the mutants containing different FeSO4 concentrations (0, 100, 500, 1000, 5000, and 10,000 μM). The strains were inoculated with 5 μL on each CSM plate. b qRT-PCR analysis of the expression levels of spore-related genes. Total RNA of the wild-type and Δbfr strains was extracted separately on the fourth day. The 16S rRNA served as the normalization control. c Colony surface area statistics of the wild-type and mutant strains. Averages from three biological replicates are shown. Error bars represent the standard deviation of the mean. *, **and *** indicate P < 0.05, P < 0.01 and P < 0.005, respectively, compared to the wild-type under the same conditions

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