Table 2 Purification scheme of recombinant β-amylase
Purification steps | Total protein (mg) | Total activity (U) | Specific activity (U mg− 1) | Purification (-fold) | Yield (%) |
|---|---|---|---|---|---|
Crude Enzyme | 113.7 | 92711.0 | 815.4 | 1 | 100 |
Ammonium Sulfate Fraction | 53.6 | 69927.8 | 1304.6 | 1.6 | 75.4 |
Superdex™ 200 Increase 10/300 GL | 2.3 | 8737.5 | 3798.9 | 4.7 | 9.4 |