Primer | Sequence(5′-3′) | Restriction sites |
---|---|---|
GFP-F | GCGCATATGAGCAAAGGAGAAGAAC | NdeI |
GFP-R | ATAGGATCCTTAGTATAGCTCATCCATG | BamHI |
FaeLam-F | TATACATATGTCCCGCATTACAATTG | NdeI |
FaeLam-R-His6 | GCGCTCGAGTTAGTGGTGGTGGTGGTGGTGGAATAATGGTTTTAAAAATT | XhoI |
S106A-F | GTCATGCTCAAGGCGGCG | Â |
S106A-R | CGCCGCCTTGAGCATGACCTACTAAAAAGATATTGC | Â |
FaeLamΔ20-F | ATACATATGTTTGGCGAAATTTATGACATG | NdeI |
CBM-F1 | ACACTTACGATTGGAGGCAG | Â |
CBM-F2 | ATACATATGACACTTACGATTGGAGGCAG | NdeI |
CBM-R-His6 | ATACTCGAGTTAGTGATGATGATGATGATGATGGATGTCCAAATAGTC | XhoI |
N20CBM-R | GCCTCCAATCGTAAGTGTAGGCTCTTCACGATCTCC | Â |
FaeLamCBM-R | GCCTCCAATCGTAAGTGTCTAGAATAATGGTTTT | Â |