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Table 3 The primers used in this study

From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coli

Primer

Sequence(5′-3′)

Restriction sites

GFP-F

GCGCATATGAGCAAAGGAGAAGAAC

NdeI

GFP-R

ATAGGATCCTTAGTATAGCTCATCCATG

BamHI

FaeLam-F

TATACATATGTCCCGCATTACAATTG

NdeI

FaeLam-R-His6

GCGCTCGAGTTAGTGGTGGTGGTGGTGGTGGAATAATGGTTTTAAAAATT

XhoI

S106A-F

GTCATGCTCAAGGCGGCG

 

S106A-R

CGCCGCCTTGAGCATGACCTACTAAAAAGATATTGC

 

FaeLamΔ20-F

ATACATATGTTTGGCGAAATTTATGACATG

NdeI

CBM-F1

ACACTTACGATTGGAGGCAG

 

CBM-F2

ATACATATGACACTTACGATTGGAGGCAG

NdeI

CBM-R-His6

ATACTCGAGTTAGTGATGATGATGATGATGATGGATGTCCAAATAGTC

XhoI

N20CBM-R

GCCTCCAATCGTAAGTGTAGGCTCTTCACGATCTCC

 

FaeLamCBM-R

GCCTCCAATCGTAAGTGTCTAGAATAATGGTTTT

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