Plasmids | Description | Source |
---|---|---|
pUC19 | Ampr, ori | Novagen |
pD403 | 2.8Â kb cryptic plasmid of L. planturum D403 | Our laboratory |
pET22b-FaeLam | Ampr, pET-22b vector ligated with faeLam gene | Our laboratory |
pNZ8148 | Cmr | Mierau and Kleerebezem [41] |
pMN402 | Derivation of gfp gene | Scholz et al. [15] |
pLP3804 | Cmr, ori from pUC19, ori and repA from pD403, promoter Ptuf | This study |
pLP-GFP | pLP3804, gfp gene under promoter Ptuf | This study |
pLP-FaeLam | pLP3804, faeLam gene under promoter Ptuf | This study |
pLP-Δ20FaeLam | pLP3804, faeLam gene that truncated 60 bp at 5′region | This study |
pLP-FaeLamS106A | pLP3804, the mutated faeLam gene under promoter Ptuf | This study |
pLP-CBM | pLP3804, cbm from Paenibacillus panacisoli under promoter Ptuf | This study |
pLP-N20CBM | pLP3804, n20 gene fusion with cbm from Paenibacillus panacisoli | This study |
pLP-FaeLamCBM | pLP3804, faeLam gene fusion with cbm from Paenibacillus panacisoli | This study |