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Table 2 The plasmids used in this study

From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coli

Plasmids

Description

Source

pUC19

Ampr, ori

Novagen

pD403

2.8 kb cryptic plasmid of L. planturum D403

Our laboratory

pET22b-FaeLam

Ampr, pET-22b vector ligated with faeLam gene

Our laboratory

pNZ8148

Cmr

Mierau and Kleerebezem [41]

pMN402

Derivation of gfp gene

Scholz et al. [15]

pLP3804

Cmr, ori from pUC19, ori and repA from pD403, promoter Ptuf

This study

pLP-GFP

pLP3804, gfp gene under promoter Ptuf

This study

pLP-FaeLam

pLP3804, faeLam gene under promoter Ptuf

This study

pLP-Δ20FaeLam

pLP3804, faeLam gene that truncated 60 bp at 5′region

This study

pLP-FaeLamS106A

pLP3804, the mutated faeLam gene under promoter Ptuf

This study

pLP-CBM

pLP3804, cbm from Paenibacillus panacisoli under promoter Ptuf

This study

pLP-N20CBM

pLP3804, n20 gene fusion with cbm from Paenibacillus panacisoli

This study

pLP-FaeLamCBM

pLP3804, faeLam gene fusion with cbm from Paenibacillus panacisoli

This study