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Table 1 The intracellular and extracellular feruloyl esterase activities (U/mL) in different recombinant strains

From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coli

Feruloyl esterase activities

L. plantarum CGMCC6888

With pLP3804-FaeLam

E. coli DH5α

With pLP3804-FaeLam

E. coli BL21(DE3)

With pET22b-FaeLam

Intracellular

397.1 ± 19.4

1742.3 ± 46.7

3886.4 ± 71.2

Extracellular

ND

540.9 ± 25.6

2013.7 ± 44.3

  1. Feruloyl esterase activity was determined by using ρNPF as substrate. ND means no detectable activity via this method