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Fig. 5 | Microbial Cell Factories

Fig. 5

From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coli

Fig. 5

The translocation and activity of heterologous protein fused with FaeLam or its N-terminus. A Western blot analysis of the subcellular localization of CBM, N20CBM and FaeLamCBM expressed in L. plantarum CGMCC6888; B Western blot analysis of the subcellular localization of CBM, N20CBM and FaeLamCBM expressed in E. coli DH5α; C SDS-PAGE analysis of the binding abilities of N20CBM, FaeLamCBM and FaeLam to de-starched wheat bran. I: intracellular fraction; E: extracellular fraction; −: unbound fraction; +: fraction bound to de-starched wheat bran

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Microbial Cell Factories

ISSN: 1475-2859

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