Fig. 5From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coliThe translocation and activity of heterologous protein fused with FaeLam or its N-terminus. A Western blot analysis of the subcellular localization of CBM, N20CBM and FaeLamCBM expressed in L. plantarum CGMCC6888; B Western blot analysis of the subcellular localization of CBM, N20CBM and FaeLamCBM expressed in E. coli DH5α; C SDS-PAGE analysis of the binding abilities of N20CBM, FaeLamCBM and FaeLam to de-starched wheat bran. I: intracellular fraction; E: extracellular fraction; −: unbound fraction; +: fraction bound to de-starched wheat branBack to article page