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Fig. 4 | Microbial Cell Factories

Fig. 4

From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coli

Fig. 4

Activity assay and subcellular localization of Δ20FaeLam. A E. coli DH5α containing pLP-Δ20FaeLam grown in LB medium supplementary with ethyl ferulate. B Western blot analysis of the extracellular proteins of E. coli DH5α containing pLP-FaeLam and pLP-Δ20FaeLam. C SDS-PAGE analysis of the whole cell protein and soluble cell extracts of E. coli DH5α containing pLP3804-FaeLam and pLP3804-Δ20FaeLam. M: Marker (from top to bottom: 116, 66.2, 45, 35, 25, 18.4, 14.4 kDa)

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