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Fig. 3 | Microbial Cell Factories

Fig. 3

From: The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia coli

Fig. 3

Activity assay and subcellular localization of FaeLamS106A. A L. plantarum CGMCC6888 containing pLP-FaeLamS106A grown in MRS medium supplementary with ethyl ferulate; B Western blot analysis of the subcellular localization of FaeLamS106A expressed in L. plantarum CGMCC6888; C E. coli DH5α containing pLP-FaeLamS106A grown in LB medium supplementary with ethyl ferulate; D Western blot analysis of the subcellular localization of FaeLamS106A expressed in E. coli DH5α. I: intracellular fraction; E: extracellular fraction

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