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Table 1 Primers used in this study

From: Production of neoagarooligosaccharides by probiotic yeast Saccharomyces cerevisiae var. boulardii engineered as a microbial cell factory

Primer

Sequence (5′→3′, restriction sites are underlined)

gHIS3_F

TCCACCTAGCGGATGACTCT

gHIS3_R

TGCATTACCTTGTCATCTTC

dDNA_HIS3_F

GTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTTAACCC

dDNA_HIS3_R

TTCTGGGAAGATCGAGTGCTCTATCGCTAGGGGTTAACCCTTTAAAGAGATCGCAATCTG

16A_W/OSP_F_SpeI

ATA ACT AGT GCA GAA AAT TTA AAT AAT AAA TCA TAC GAG TG

16A_W/OSP_R_XhoI

ATA CTC GAG TTC TTC TGG GAC CAG TGT ATA AAC

16A_CL_F_SpeI

ATA ACT AGT ATG AGG TCT TTG CTA ATC TTG GTG CTT TGC TTC CTG CCC CTG GCT GCT CTG GGG GCA GAA AAT TTA AAT AAT AAA TCA TAC

16A_CL_R_XhoI

ATA CTC GAG TTC TTC TGG GAC CAG T

αMF_F_SpeI

ATA ACT AGT ATG AGA TTT CCT TCA ATT TTT ACT G

αMF_R_16A

GCA GAA AAT TTA AAT AAT AAA GCT TCA GCC TCT CTT TTC T

16A_F_ αMF

GAG AAA AGA GAG GCT GAA GCT GCA GAA AAT TTA AAT AAT AAA TCA TAC G

16A_R_αMF_BamHI

ATA GGA TCC TTC TTC TGG GAC CAG TGT AT

STA1_F_EcoRI

ATA GAA TTC ATG GTA GGC CTC AAA AAT C

STA1_16A_ R

GCA GAA AAT TTA AAT AAT AAA TCA TAC GAG TTT TTT CTG TCG CTG GAG C

16A_STA1_ F

GGC TCC AGC GAC AGA AAA AAG CAG AAA ATT TAA ATA ATA AAT CAT ACG AG

16A_ R_XhoI

ATA CTC GAG TTC TTC TGG GAC CAG TGT AT

16A_SED1_F_SpeI

ATA ACT AGT ATG AAA TTA TCA ACT GTC CTA TTA TCT GCC GGT TTA GCC TCG ACT ACT TTG GCC CAA GCA GAA AAT TTA AAT AAT AAA TCA

16A_SED1_R_XhoI

ATA CTC GAG TTC TTC TGG GAC CAG

dDNA-CS5-F

AAA AGA GAA GAA AAA AGA GAA GAA ATG AAT TCT ATT ATG ATA GCG AAT GCA ATT AAC CCT CAC TAA AGG GA

dDNA-CS5-R

TGC TGG TTG CCT TAT TAA TTT ATA TGG AAG ACG AGA TAA TTC ATT AAT TAG TAA TAC GAC TCA CTA TAG GGC

dDNA-CS5 + 60_F

ATG GTA CAC GCT CTT GGC AAC ATT GAA ATT ACA GCT CTC ATA TAT AAA AAA TGG AAA GAA AAA AGA GAA GAA AAA AGA GAA GAA ATG AAT

dDNA-CS5 + 60_R

GGC ATA ACA ATA GCG CAC AGA TCC GCA GGT TTC GTA ATA CGC TTA ACA ATA GGC GTC TCC TGC TGG TTG CCT TAT TAA TTT ATA TGG AAG

gCS5_F

CTG GTA GTT GCA CAG AAA GAG TTT TAG AGC TAG AAA TAG CAA G

gCS5_R

TCT TTC TGT GCA ACT ACC AGC GAT CAT TTA TCT TTC ACT GCG

Conf-CS5-F

AAT GAA TTC TAT TAT GAT AGC GAA TGC

Conf-CS5-R

CAC AGG ATT TAC GAA GAC C