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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Production of neoagarooligosaccharides by probiotic yeast Saccharomyces cerevisiae var. boulardii engineered as a microbial cell factory

Fig. 4

Fermentation profiles of engineered S. boulardii SB-HTU_16A_D harboring p426_Bp_SED1. A Time course analysis of the fermentation products of SB-HTU_16A_D using TLC. B Fermentation profiles of engineered S. boulardii SB-HTU_16A_D in YSC medium containing 20 g/L glucose and 2.5 g/L agarose in 50 mM KHP buffer (pH 5.5) at 37 °C and 200 rpm for 72 h. During fermentation, the cell density (OD600) and the concentration values of glucose, ethanol, acetic acid, and NeoDP4 were monitored using HPLC. All data points are the means of experimental data from triplicate fermentations. SB-HTU_16A_D harboring p426_Bp_SED1. NeoDP4, neoagarotetraose; NeoDP2, neoagarobiose; YSC, yeast synthetic complete

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