Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of neoagarooligosaccharides by probiotic yeast Saccharomyces cerevisiae var. boulardii engineered as a microbial cell factory

Fig. 3

Signal peptide screening for the production of NeoDP4 in S. boulardii. A TLC analysis of the fermentation products by engineered yeast strains. B Comparison of the extracellular NeoDP4 concentration depending on the origin of the signal peptide sequences. Empty, SB-HTU_16A_E harboring pRS426GPD; W/OSP, SB-HTU_16A_W harboring p426_Bp_W/OSP; CL, SB-HTU_16A_C harboring p426_Bp_CL; α-MF, SB-HTU_16A_A harboring p426_Bp_αMF; STA1, SB-HTU_16A_S harboring p426_Bp_STA1; SED1, SB-HTU_16A_D harboring p426_Bp_SED1. Engineered strains were cultured with 20 g/L glucose and 2.5 g/L agarose in 50 mM KHP buffer (pH 5.5) at 37 °C and 200 rpm for 72 h. Results are presented as the mean values and standard deviations of data from three independent biological replicates. NeoDP4, neoagarotetraose; NeoDP2, neoagarobiose

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us