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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Vanillic acid and methoxyhydroquinone production from guaiacyl units and related aromatic compounds using Aspergillus niger cell factories

Fig. 5

Analysis of MhdA activity by HPLC and identification of 4-hydroxy-6-methoxy-6-oxohexa-2,4-dienoic acid by Orbitrap LC–HRMS product. a Chromatogram of methoxyhydroquinone incubated with and without MhdA. The reaction mixture was incubated at 30 °C for 1 h. b Total ion chromatograms (TIC) of the ethyl acetate extracted reaction mixture of methoxyhydroquinone incubated with MhdA and the mass spectra of product 4-hydroxy-6-methoxy-6-oxohexa-2,4-dienoic acid peak at retention time 3.78 min at negative and positive ionization mode

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