Table 3 Scale up of SP Sepharose FastFlow column
From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
Parameter | Small scale | Higher scale |
|---|---|---|
Column hardware | Glass column 15 × 125, Kronlab | XK26, GE Healthcare |
Radius | 0.75 cm | 1.3 cm |
Bed volume | 5 mL | 20 mL |
Bed height | 2.9 cm | 3.76 cm |
Volumetric flow rate | 3 mL min− 1 | 9.04 mL min− 1 |
Linear flow velocity | 1.7 cm min− 1 | 1.7 cm min− 1 |