Fig. 7From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purificationApplication of RNase PF in a plasmid purification process. Cleared alkaline lysates without or with added RNase (bovine RNase A or RNase PF) were compared for removal of the RNA cloud. The lysates were then purified by a proprietary DNA purification chromatography step. The plasmid eluates from the three cases were compared on the basis of residual RNA. – and + refer to before and after treatment with RNase and incubation at 37 °C for 15 min. For control, no RNase was addedBack to article page