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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification

Fig. 4

Chromatogram from a Toyopearl 650 M Butyl hydrophobic interaction chromatography of a RNase activity-containing elution fraction from a cation exchange chromatography step, prepared for HIC by mixing with 3 M ammonium sulphate. Inlay: RNase activity test of various fractions. S: RNA substrate, N: Negative control, E: Elution from cation exchange chromatography, Ed: Elution from cation exchange chromatography diluted, FT: Flowthrough fraction, W: Wash, E1–E6: Elution fractions. The high concentration of ammonium sulphate causes smearing and RNA band displacement in the elution fractions

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