Fig. 2
From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
Chromatogram from a SP Sepharose FastFlow cation exchange chromatography of the retentate fraction from tangential flow filtration. The observation from Fig. 1 could be reproduced here including additional information about the buffer pH (see text for details). Inlay: RNase activity test of various fractions. S: RNA substrate, N: Negative control, R: Retentate after dialysis, Rd: Retentate after dialysis and diluted with 10 mM sodium acetate buffer, FT: Flowthrough fraction, E1-E4: Elution fractions from cation exchange chromatography, CIP: Cleaning-In-Place fraction