Fig. 1From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purificationChromatogram from a SP Sepharose FastFlow cation exchange chromatography of the retentate fraction from tangential flow filtration. Inlay: RNase activity test of various fractions. S: RNA substrate, N: Negative control, P: Permeate from TFF, FT: Flowthrough fraction, E1-E5: Elution fractions, CIP: Cleaning-In-Place fraction from cation exchange chromatographyBack to article page