Fig. 4

Modulation of tHMG1 expression using chimeric promoter variants. A Schematic diagram of the strategy used to construct the tHMG1 Gene expression cassette under the control of different chimeric promoters using CRISPR-aided homologous recombination. B Squalene production by engineered S. cerevisiae overexpressing the tHMG1 gene using the K₀, K₅₄₀, K₅₃₆, and K₅₂₈ chimeric promoter variants after 10 days of cultivation