Skip to main content
Fig. 2 | Microbial Cell Factories

Fig. 2

From: Fine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants

Fig. 2

Selection and verification of the UASF-E-C-Core1 artificial promoter as library template. A Comparison of the strength of the UASF-E-C-Core1 promoter with known strong promoters of S. cerevisiae by measuring the OD600-adjusted GFP fluorescence intensity. B A schematic diagram of the components of the UASF-E-C-Core1 promoter with extended or shortened 5′ UTR sequences. C The OD600-adjusted GFP fluorescence intensity for the original, 5’UTR-extended, and 5’UTR-shortened UASF-E-C-Core1 promoters. All data represent the means ± SD of biological triplicates. The significance of differences was assessed using one-way ANOVA. *P < 0.01; N.S., not significant

Back to article page