Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Fine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants

Fig. 1

A general workflow employed in the current study. The chimeric promoter library was generated by randomizing the hexameric Kozak motif of the UASF-E-C-Core1 artificial promoter to any of the four DNA bases. Several Kozak variants with strong protein expression activity were obtained by GFP fluorescence screening. This library was then applied for metabolic pathway optimization in S. cerevisiae BY4742 strain

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us