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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Expression of the human molecular chaperone domain Bri2 BRICHOS on a gram per liter scale with an E. coli fed-batch culture

Fig. 3

SDS-PAGE analysis of the rh Bri2 BRICHOS species isolated with size exclusion chromatography. E1 indicates the eluted NT*-Bri2 BRICHOS from IMAC. Oligomer (oli), dimer (dim) and monomer (mon) fractions labeled with ‘cleavage’, correspond to the SEC isolated NT*-Bri2 species after overnight thrombin cleavage. After individual cleavage, each species was purified with reverse IMAC to remove the NT*-tag, followed by SEC, to isolate the oligomer, tetramer, dimer, and monomers. The individual species were collected and analyzed by SDS-PAGE under reducing (red.) and non-reducing (non-red.) conditions. M indicates the protein ladder. Noteworthy, the tetramer (tet.) fraction also contains a small amount of hexamer, which could have formed after collection of the corresponding peak eluted from SEC. The dimer band visible in the same fraction could either be a contamination from the adjacent dimer peak or emanate from a non-covalent tetramer

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Microbial Cell Factories

ISSN: 1475-2859

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