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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Expression of the human molecular chaperone domain Bri2 BRICHOS on a gram per liter scale with an E. coli fed-batch culture

Fig. 2

Size exclusion chromatography of NT*-Bri2 BRICHOS and Bri2 BRICHOS after cleavage with thrombin and reverse IMAC. a Chromatogram of NT*-Bri2 BRICHOS using a Superdex 200 column to isolate the individual oligomeric species (monomer: peak IV, dimer: peak III, tetramer: peak II and higher oligomers: peak I). The individual species were then cleaved with thrombin to remove the NT*-tag and Bri2 BRICHOS was separated from the NT*-tag using reverse IMAC. b Chromatograms of oligomeric (I), tetrameric (II) and dimeric (III) Bri2 BRICHOS using a Superdex 200 column. c Chromatogram of monomeric (IV) Bri2 BRICHOS using a Superdex 75 column

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