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Fig. 6 | Microbial Cell Factories

Fig. 6

From: The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production

Fig. 6

a Bright-field images (left) and fluorescence microscopy (right) of Fluo-3 AM-stained mycelia (×400) treated by vitamin C (Vc) or diphenyleneiodonium chloride (DPI) in the co-culture. b Ca2+ accumulation in S9 treated by Vc or DPI in the co-culture. Vc (0.1 mM) or DPI (5 µM) was added to the culture at 1 h prior to the addition of SB1 (400 cells/mL). c Bright-field images (left) and fluorescence microscopy (right) of 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA)-stained mycelia treated by EGTA (5 mM) and La3+ (×400). d H2O2 content of S9 treated by EGTA or La3+ in the co-culture. EGTA (5 mM) and La3+ (2 mM) were added to the culture at 1 h prior to the addition of SB1 (400 cells/mL). Values are mean ± SD from three independent experiments (**p < 0.01 vs. control)

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