Fig. 5

Effect of eATP on SB1-induced Ca²⁺ influx of Shiraia sp. S9. a Bright-field images (left) and fluorescence microscopy (right) of Fluo-3 AM-stained mycelia (400 ×). b Ca²⁺ accumulation in S9 after the addition of SB1 at 400 cells/mL. c Bright-field images (left) and fluorescence microscopy (right) and d fluorescent intensity of Fluo-3 AM-stained mycelia (400 ×) treated by pyridoxalphosphate-6-azophenyl-2′, 4′-disulfonic acid (PPADS), reactive blue (RB) and apyrase in the co-culture. SB1 treatment was the same as specified in Fig. 1. The dosages of PPADS, RB and apyrase were the same as specified in Fig. 2. Values are mean ± SD from three independent experiments (**p < 0.01 vs. control, ^##p < 0.01 vs. SB1 treatment)