Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production

Fig. 4

Effect of eATP on SB1-induced ROS accumulation of Shiraia sp. S9. a Bright-field images (left) and fluorescence microscopy (right) of 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA)-stained mycelia (×400). b Reactive oxygen species (ROS) accumulation in S9 after the addition of SB1 at 400 cells/mL. c The effect of pyridoxalphosphate-6-azophenyl-2′, 4′-disulfonic acid (PPADS), reactive blue (RB) and apyrase on SB1-induced ROS accumulation and d H2O2 content of S9. The 6-day-old fungal mycelia were incubated with 10 µM DCFH-DA for 30 min. The SB1 treatment was the same as specified in Fig. 1. The dosages of PPADS, RB and apyrase were the same as specified in Fig. 2. Values are mean ± SD from three independent experiments (**p < 0.01 vs. control)

Back to article page