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Fig. 2 | Microbial Cell Factories

Fig. 2

From: The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production

Fig. 2

The effect of ATP on hypocrellin A (HA) production of Shiraia sp. S9. a The effects of exogenous ATP (exATP) on the growth and red pigments secretion of Shiraia sp. S9 in PDA plate. S9 was treated with exATP and incubated at 28 °C for 10 days. b The effects of exATP on HA production in submerged culture. exATP was added on day 6 during the 8-day-culture of S9. c The effect of purinoceptor inhibitors and apyrase on SB1-induced HA production. The 6-day-old fungal mycelia were incubated with inhibitors pyridoxalphosphate-6-azophenyl-2′, 4′-disulfonic acid (PPADS) and reactive blue (RB) at 10 µM and apyrase (2 U/mL) for 1 h before SB1 addition and cultured for additional 2 days. d The effects of ATP analog (ATPγS) and derivatives (ADP and AMP) on HA production in submerged culture. exATP, ATPγS, ADP and AMP were added respectively at 100 µM on day 6 during the 8-day-culture of S9. Values are mean ± SD from three independent experiments (*p < 0.05 and **p < 0.01 vs. control, #p < 0.05 and ##p < 0.01 vs. SB1 treatment). Different letters above the bars mean significant differences (p < 0.05)

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