Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: The signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production

Fig. 1

Hypocrellin A (HA) production and ATP release in the co-culture of Shiraia sp. S9 and P. fulva SB1. a Scheme of the in vitro dual culture plate confrontation assay. b The effects of live SB1 on the growth and red pigments secretion of S9 in PDA plate. The 4-day-old fungal colony was treated with SB1 for 2–6 days. The bacterial suspension (10 µL) was streaked in two parallel straight lines, approximately 7 cm apart from each other. The culture was maintained on PDA at 28 °C. c The total HA production of S9 in the co-culture. The co-culture was maintained in 150-mL flask containing 50 mL of the liquid medium at 150 rpm and 28 °C for 8 d. d ATP release in the co-culture. The 6-day-old fungal mycelia were treated with SB1 (400 cells/mL) and incubated at 150 rpm and 28 °C. Apyrase at 2 U/mL was added to the culture at 1 h prior to the addition of SB1. e The released ATP in the fungal culture with live SB1 (B, 400 cells/mL), heat-killed SB1 (DB, 400 cells/mL), the crude bacterial polysaccharide (BPS) and bacterial broth extracts (BBE) at 100 mg/mL for 1 h. f The released ATP in bacterial culture with fungal polysaccharides (FPS) and the ethyl acetate extracts (FBE) at 100 mg/mL and HA (5 mg/mL) treatment for 1 h. Values are mean ± SD from three independent experiments (*p < 0.05 and **p < 0.01 vs. control). Different letters above the bars mean significant differences (p < 0.05)

Back to article page