Table1 Plasmids used in this study
From: Development and optimization of a microbial co-culture system for heterologous indigo biosynthesis
Plasmid | Description | Source |
|---|---|---|
pET28a | T7 promoter, KanR | Novagen |
pACYCDuet-1 | Double T7 promoters, CmR | Novagen |
pET21c | T7 promoter, AmpR | Novagen |
pCDFDuet-1 | T7 promoter, StrpR | Novagen |
pTE3 | pET28a carrying the trpEfbr and aroGfbr genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc) | [21] |
pTD2 | pACYC Duet carrying the trpD, trpC, trpB and trpA genes under the control of the constitutive Zymomonas mobilis pyruvate decarboxylase promoter (Ppdc) | [21] |
pSE | pET21c carrying tryptophan regulatory element (tnaC operon) and the tetA gene, AmpR | [16] |
pSE1 | pET21c carrying the hipA gene under the control of the mtr promoter (Pmtr) | [21] |
pET28a carrying the fmo gene (from Methylophaga aminisulfidivorans) under control of T7 promoter | This study | |
pMF | pCDFDuet-1 carrying the fmo gene (from Methylophaga aminisulfidivorans) under control of T7 promoter | This study |
pDT | pET21c carrying the tetA gene under the control of the tetA promoter (Ptet) | This study |
pTS0 | pET28a carrying the proA promoter with the kanR gene replaced by the strpR gene | This study |
pTS1 | pTS0 carrying rpoA gene under control of proA promoter | This study |
pTS2 | pTS1 after random mutagenesis | This study |