Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

Table 5 Amino acid sequences and the key physicochemical parameters

Peptide	Sequences	Theoretical MW (Da)	Measured MW (Da)^a	Net charge^b	H^b
FD	FKRIVQRIKDFLR	1719.11	1719.25	+ 4	0.315
FR	FKRIVQRIKRFLR	1759.19	1759.00	+ 6	0.297
(FR)₂	FKRIVQRIKRFLRFKRIVQRIKRFLR	3502.38	3503.10	+ 12	0.297
LG	FKRIVQRIKRFLRGGGGSFKRIVQRIKRFLR	3817.69	3817.93	+ 12	0.248
LA	FKRIVQRIKRFLRAEAAAKAFKRIVQRIKRFLR	4115.06	4115.60	+ 12	0.232

^aMolecular weight (MW) was measured by mass spectroscopy (MS)
^bThe net charge and the values of the mean hydrophobicity (H) values were calculated from http://heliquest.ipmc.cnrs.fr/cgi-bin/ComputParams.py

ISSN: 1475-2859