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Fig. 9 | Microbial Cell Factories

Fig. 9

From: A cell engineering approach to enzyme-based fed-batch fermentation

Fig. 9

PcstA regulated C. violaceum glucoamylase expression and its influence on growth in MSM containing starch–agar layers. E. coli W3110 harbouring pQR1715 (BBa_K118011-CV2DsbA) cultured for 72 h at 30 °C, 180 rpm, in either glucose free MSM with starch–agar layers (circle) or MSM supplemented with 50 g/L glucose (square). a Growth curves for each condition in duplicate. b Glucoamylase activity in the extracellular media determined by amylase assay at 37 °C and expressed as units per mL of culture. c Glucoamylase activity in the extracellular media determined by amylase assay at 37 °C and expressed as units per gram of dry cell weight. Units defined as the disappearance of 1 mg/mL of starch/iodine complex per minute, determined by the change in absorbance of starch/iodine complex at 600 nm

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