Fig. 8
From: A cell engineering approach to enzyme-based fed-batch fermentation
Characterisation of PcstA regulated C. violaceum glucoamylase secretion in LB media supplemented with 0–20 mM glucose. Extracellular glucoamylase activity of E. coli W3110 harbouring pQR1715 (BBa_K118011-CV2DsbA) cultured for 24 h at 37 °C, 250 rpm, in LB containing a range of glucose concentrations (0–20 mM). Activity expressed as units (defined as the disappearance of 1 mg/mL of starch/iodine complex per minute) per gram of dry cell weight and determined by the change in absorbance of starch/iodine complex at 600 nm. Error bars represent standard deviation, n = 3