Fig. 5
From: A cell engineering approach to enzyme-based fed-batch fermentation
Relative activity of C. violaceum glucoamylase with temperature. Extracellular activity of C. violaceum glucoamylase at varying temperatures (20 °C to 60 °C) expressed as a percentage of the activity from the standard amylolytic assay carried out at 37 °C. Extracellular media samples for assays extracted from BL21(DE3) harbouring pQR1705 (C. violaceum glucoamylase) cultured in LB for 24 h at 25 °C, 180 rpm (induced with 0.4 mM IPTG at an OD600 of 0.6–0.8). Activities within standard amylase assay determined by the change in absorbance of starch/iodine complex at 600 nm. Error bars represent standard deviation, n = 3