Fig. 2
From: A cell engineering approach to enzyme-based fed-batch fermentation
Starch degradation and glucose accumulation from cell free extracts of D. geothermalis, T. tengcongensis and C. violaceum glucoamylases. Cell free extracts from BL21(DE3) harbouring either pQR1706 (D. geothermalis glucoamylase, pQR1707 (T. tengcongensis glucoamylase), or pQR1708 (C. violaceum glucoamylase), induced with 0.4 mM IPTG at an OD600 of 0.8 and grown in TB for 24 h at 25 °C, 180 rpm. a Concentration (mg/mL) of remaining starch (dark grey) and accumulated glucose (light grey) following 1 h of amylase assay for each CFE. b Starch degrading activity in units (dark grey) defined as the disappearance of 1 mg/mL of starch/iodine complex per minute per gram of dry cell weight, and glucose accumulating activity in units (light grey) defined as accumulation of 1 mg/mL of glucose per minute per gram of dry cell weight, for each CFE. Starch degradation in amylase assay determined by the change in absorbance of starch/iodine complex at 600 nm, glucose accumulation measured using HPAEC-PAD analysis. Error bars represent standard deviation, n = 3