Fig. 11
From: A cell engineering approach to enzyme-based fed-batch fermentation
Vector designed for simultaneous expression of eGFP and C. violaceum glucoamylase. Vector map of pQR1720, constructed using multiple insert CPEC following insert and vector PCR reactions using the primer pairs indicated. Insert 1 (eGFP) amplified using CPEC8_GFP.rv and CPEC9_GFP.fw primers, with pQR1344 as the template, insert 2 (C. violaceum glucoamylase with attached DsbA signal sequence) amplified using CPEC9_CVprimer2Dsb.rv and CPEC9_CVprimer2Dsb.fw primers, with pQR1712 as the template, and the vector backbone amplified using CPEC9_pUC19.rv and CPEC8_pUC19.fw primers, with pUC19 as the template