Fig. 10
From: A cell engineering approach to enzyme-based fed-batch fermentation
Self secreting enzyme-based fed-batch fermentation via simultaneous expression of C. violaceum glucoamylase and eGFP in E. coli W3110. E. coli W3110 harbouring pQR1720, cultured in flasks containing glucose free MSM with starch–agar layers or MSM supplemented with 50 g/L glucose, at 37 °C, 250 rpm for 48 h [induced with 0.4 mM IPTG at inoculation (initial OD600 of 0.25)]. a Increase in cell density (OD600) over 48 h of culture for each media type. b Increase in extracellular media glucoamylase activity over 48 h for each media type, expressed in units of glucoamylase activity per mL of culture, determined by amylase assay at 37 °C and expressed as units per mL of culture. Units defined as the disappearance of 1 mg/mL of starch/iodine complex per minute, determined by the change in absorbance of starch/iodine complex at 600 nm. c Increase in total eGFP fluorescence units over 48 h for each media type, measured from neat whole cell samples using an excitation wavelength of 483 nm and emission wavelength of 535 nm (Tecan infinite 200 pro). d Image of eGFP expression under blue light for each media composition, displayed in triplicate. Error bars represent standard deviation, n = 3